pCMV-Myc-XPLN-L269E
(Plasmid
#73376)
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PurposeMammalian expression of mutant XPLN (Arhgef3) gene Leu269 --> Glu
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 73376 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMV-Myc
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namearhgef3
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Alt nameXPLN
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SpeciesH. sapiens (human)
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MutationL269E
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Entrez GeneARHGEF3 (a.k.a. GEF3, STA3, XPLN)
- Promoter CMV
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Tag
/ Fusion Protein
- Myc (QKLISEEDL) (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EBV-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-Myc-XPLN-L269E was a gift from Jie Chen (Addgene plasmid # 73376 ; http://n2t.net/addgene:73376 ; RRID:Addgene_73376) -
For your References section:
XPLN is an endogenous inhibitor of mTORC2. Khanna N, Fang Y, Yoon MS, Chen J. Proc Natl Acad Sci U S A. 2013 Oct 1;110(40):15979-84. doi: 10.1073/pnas.1310434110. Epub 2013 Sep 16. 10.1073/pnas.1310434110 PubMed 24043828