pCMV-Myc-XPLN
(Plasmid #73365)

• Purpose: Expresses myc tagged XPLN in mammalian cells
• Depositing Labs: Jie Chen, Krister Wennerberg
• Publication: Arthur et al J Biol Chem. 2002 Nov 8;277(45):42964-72. Epub 2002 Sep 6.

Backbone

• Vector backbone: pCMV-Myc
• Backbone manufacturer: clontech
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: arhgef3
• Alt name: XPLN
• Species: H. sapiens (human)
• Entrez Gene: ARHGEF3 (a.k.a. GEF3, STA3, XPLN)
• Promoter: CMV
• Tag / Fusion Protein:
  • Myc (QKLISEEDL) (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EBV-rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Received as gift from Professor Krister Wennerberg (University of Helsinki, Helsinki).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Original Publication: XPLN, a Guanine Nucleotide Exchange Factor for RhoA and RhoB, But Not RhoC