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PurposeExpression of STAT3-S727D
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 73364 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDNA3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSignal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor)
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SpeciesM. musculus (mouse)
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MutationE16K S727D
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GenBank IDNP_998824.1
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG tagged (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
E16K mutation originated in Plasmid #8706 but does not affect function.
WT control for Plasmid 73364: pCDNA3-STAT3-S727D is Plasmid 8706: Stat3 pcDNA3 from the Darnell lab.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA3-STAT3-S727D was a gift from Jie Chen (Addgene plasmid # 73364 ; http://n2t.net/addgene:73364 ; RRID:Addgene_73364) -
For your References section:
Signal transducer and activator of transcription 3 (STAT3) mediates amino acid inhibition of insulin signaling through serine 727 phosphorylation. Kim JH, Yoon MS, Chen J. J Biol Chem. 2009 Dec 18;284(51):35425-32. doi: 10.1074/jbc.M109.051516. Epub . 10.1074/jbc.M109.051516 PubMed 19875458