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Addgene

pJK275
(Plasmid #73363)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73363 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYES2
  • Backbone size w/o insert (bp) 5857
  • Total vector size (bp) 7182
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    delta-8 sphingolipid desaturase
  • Alt name
    SLD1
  • Species
    Crepis alpina
  • Mutation
    missing residues 1-6
  • GenBank ID
    DQ176016.1
  • Promoter GAL1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer pYESTrp_rev_primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK275 was a gift from T. J. Kappock (Addgene plasmid # 73363 ; http://n2t.net/addgene:73363 ; RRID:Addgene_73363)
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