pGolt2-mCherry
(Plasmid #73358)

• Purpose: In vivo protein protein interaction studies/ expression in mammalian cells
• Depositing Lab: Michael Kreutz
• Publication: pGolt2-mCherry, expression in mammalian cell (unpublished)

Backbone

• Vector backbone: pmCherryN1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4725
• Total vector size (bp): 4812
• Modifications to backbone: This vector contains the MCS site of pmCherryN1
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: XL10-Gold Ultracompetent cells should be used for multiplication of plasmid.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TMD (trans-membrane domain) of Calneuron-2
• Alt name: calcium binding protein 7 (Cabp7)/ C-terminal region of Calneuron-2
• Species: R. norvegicus (rat)
• Insert Size (bp): 87
• GenBank ID: NM_001007730 NP_001007731
• Entrez Gene: Cabp7 (a.k.a. rCaBP7)
• Promoter: CMV immediate early promoter
• Tag / Fusion Protein:
  • mCherry (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BspEI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: Forward primer for pEGFPN1 or pmCherryN1
• 3′ sequencing primer: Reverse primer for pmCherryN

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry