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Addgene

pAdx-EF1-FLPe-P2A-tdTomato
(Plasmid #73352)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73352 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pAdenoX
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 32705
  • Total vector size (bp) 36968
  • Vector type
    Adenoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    CMV-iCre-P2A-tdTomato
  • Insert Size (bp)
    4264
  • Promoter EF-1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site I-CeuI (not destroyed)
  • 3′ cloning site PI-SceI (not destroyed)
  • 5′ sequencing primer AATCTTACTCGGTTACGCCC
  • 3′ sequencing primer CGGCTGCTGCAAAACAGATAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAdx-EF1-FLPe-P2A-tdTomato was a gift from Kazuhiro Oka (Addgene plasmid # 73352)
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