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Addgene

pGEX-2T-FRB-W2027F
(Plasmid #73341)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73341 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGEX-2T
  • Backbone manufacturer
    Amersham Pharmacia Biotech
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    FKBP12-rapamycin binding (FRB) domain of MTOR
  • Alt name
    Mtor, FRAP
  • Species
    H. sapiens (human)
  • Mutation
    FRB domain only aa2015-2114; W2027F
  • Entrez Gene
    MTOR (a.k.a. FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS)
  • Tag / Fusion Protein
    • GST-tagged (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-2T-FRB-W2027F was a gift from Jie Chen (Addgene plasmid # 73341 ; http://n2t.net/addgene:73341 ; RRID:Addgene_73341)
  • For your References section:

    The FKBP12-rapamycin-binding domain is required for FKBP12-rapamycin-associated protein kinase activity and G1 progression. Vilella-Bach M, Nuzzi P, Fang Y, Chen J. J Biol Chem. 1999 Feb 12. 274(7):4266-72. 10.1074/jbc.274.7.4266 PubMed 9933627