pGEX-2T-FRB-W2027F
(Plasmid
#73341)
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PurposePoint mutation Trp 2027 → Phe in the FKBP12-rapamycin binding (FRB) domain of mTOR
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 73341 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX-2T
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Backbone manufacturerAmersham Pharmacia Biotech
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFKBP12-rapamycin binding (FRB) domain of MTOR
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Alt nameMtor, FRAP
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SpeciesH. sapiens (human)
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MutationFRB domain only aa2015-2114; W2027F
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Entrez GeneMTOR (a.k.a. FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS)
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Tag
/ Fusion Protein
- GST-tagged (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pGEX-5
- 3′ sequencing primer pGEX-3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX-2T-FRB-W2027F was a gift from Jie Chen (Addgene plasmid # 73341 ; http://n2t.net/addgene:73341 ; RRID:Addgene_73341) -
For your References section:
The FKBP12-rapamycin-binding domain is required for FKBP12-rapamycin-associated protein kinase activity and G1 progression. Vilella-Bach M, Nuzzi P, Fang Y, Chen J. J Biol Chem. 1999 Feb 12. 274(7):4266-72. 10.1074/jbc.274.7.4266 PubMed 9933627