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Addgene

GlgA1-Ptrc-GPPS-FNI-MK-PMD-PMK-SmR-psbA2
(Plasmid #73340)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73340 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBluescript KS+
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2905
  • Total vector size (bp) 10262
  • Selectable markers
    Streptomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GPPS-FNI-MK-PMD-PMK-SmR
  • Alt name
    Lower MVA pathway from Streptococcus pneumoniae
  • Species
    Picea abies and Streptococcus pneumoniae
  • GenBank ID
    GPPS2 (EU432047.2), MK (AAK99142.1), PMD (AAK99143.1), PMK (AAK99144.1), FNI (AAK99145.1)
  • Promoter Ptrc

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site SacI (unknown if destroyed)
  • 5′ sequencing primer GTCCCAAATTCTTGATCCCATCC
  • 3′ sequencing primer GTGGTCTTGATCGCTCGAGCTC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GlgA1-Ptrc-GPPS-FNI-MK-PMD-PMK-SmR-psbA2 was a gift from Anastasios Melis (Addgene plasmid # 73340)
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