pJK237
(Plasmid #73339)

• Purpose: Produces Acetobacter aceti 1023 citrate synthase with cleavable N-terminal His6/CBD/Ssp-intein fusion
• Depositing Lab: T. J. Kappock
• Publication: Additional Acetobacter aceti 1023 acetic acid resistance (aar) gene constructs (unpublished)

Backbone

• Vector backbone: pET28a
• Backbone size w/o insert (bp): 5369
• Total vector size (bp): 7316
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: citrate synthase
• Alt name: aarA
• Alt name: gltA
• Species: Acetobacter aceti 1023
• GenBank ID: ABF85671 KDE21011
• Promoter: T7
• Tags / Fusion Proteins:
  • His6 (N terminal on backbone)
  • chitin-binding domain (N terminal on insert)
  • Ssp intein (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Contains CBD-Ssp intein region from pTWIN-1 (NEB) with an N-terminal His tag added for alternative purification method.