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Addgene

pJK126
(Plasmid #73337)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73337 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 6401
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    electron transfer flavoprotein
  • Alt name
    fdxB
  • Alt name
    LH57_19385
  • Species
    Mycobacterium tuberculosis H37Rv
  • Mutation
    encodes residues 328-685
  • GenBank ID
    AIR16344.1
  • Promoter T7
  • Tag / Fusion Protein
    • His6 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Contains putative NAD(P)H-dependent flavoprotein oxidoreductase domain that is potentially capable of supplying electrons to the N-terminal fatty acid desaturase domain.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK126 was a gift from T. J. Kappock (Addgene plasmid # 73337 ; http://n2t.net/addgene:73337 ; RRID:Addgene_73337)
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