pLVX-FLAG-dTomato-centrin-1
(Plasmid #73332)

• Purpose: Lentiviral vector encoding centrin-1 with an N-terminal FLAG and dTomato tag
• Depositing Lab: Manuel Thery
• Publication: pLVX-EGFP-C3-Cep123 (unpublished)

Backbone

• Vector backbone: pLVX-FLAG-dTomato-C1
• Backbone manufacturer: Modified Clontech
• Backbone size w/o insert (bp): 8700
• Total vector size (bp): 9239
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: centrin-1
• Species: H. sapiens (human)
• Insert Size (bp): 519
• Entrez Gene: CETN1 (a.k.a. CEN1, CETN)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG and dTomato (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nde I (not destroyed)
• 3′ cloning site: Xho I (not destroyed)
• 5′ sequencing primer: CMV seq

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry