pEGFP-C2-Cep123-C2 domain
(Plasmid #73329)

• Purpose: Encodes the distal appendage targeting domain of isoform 1 of Cep123 (residues 500-600) fused to EGFP
• Depositing Lab: Michel Bornens
• Publication: Sillibourne et al Biol Open. 2013 Apr 9;2(6):535-45. doi: 10.1242/bio.20134457. Print 2013 Jun 15.

Backbone

• Vector backbone: pEGFP-C2
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 5019
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cep123
• Alt name: CCDC123
• Alt name: Cep89
• Species: H. sapiens (human)
• Insert Size (bp): 306
• GenBank ID: NM_032816
• Entrez Gene: CEP89 (a.k.a. CCDC123, CEP123)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH I---Bgl II (destroyed during cloning)
• 3′ cloning site: Xho I---Sal I (destroyed during cloning)
• 5′ sequencing primer: EGFP 3' Fw

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry