pJK234
(Plasmid #73324)

• Purpose: Produces Janthinobacterium lividum violacein biosynthesis proteins VioA, VioB, and VioC
• Depositing Lab: T. J. Kappock
• Publication: Janthinobacterium lividum violacein biosynthesis (unpublished)

Backbone

• Vector backbone: pET24a
• Backbone size w/o insert (bp): 5310
• Total vector size (bp): 11001
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: violacein biosynthesis protein VioA
• Alt name: vioA
• Alt name: tryptophan 2-monooxygenase
• Species: Janthinobacterium lividum
• GenBank ID: AAZ39191.1
• Promoter: T7

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: n/a (unknown if destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: n/a

Gene/Insert 2

• Gene/Insert name: violacein biosynthesis protein VioB
• Alt name: vioB
• Species: Janthinobacterium lividum
• GenBank ID: AAZ39192.1
• Promoter: n/a

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Gene/Insert 3

• Gene/Insert name: violacein biosynthesis protein VioC
• Alt name: vioC
• Alt name: monooxygenase VioC
• Species: Janthinobacterium lividum
• GenBank ID: AAZ39193.1
• Promoter: n/a

Cloning Information for Gene/Insert 3

• Cloning method: Restriction Enzyme
• 5′ cloning site: n/a (unknown if destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: T7 terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

also produces a small fragment of VioD