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Addgene

Artichoke
(Plasmid #73320)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73320 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Lentiviral
  • Backbone manufacturer
    Genetic Perturbations Platform, Broad Institute
  • Backbone size (bp) 6000
  • Vector type
    Mammalian Expression, Lentiviral
  • Promoter SFFV and EF1a
  • Selectable markers
    Puromycin
  • Tag / Fusion Protein
    • EGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Artichoke was a gift from Benjamin Ebert (Addgene plasmid # 73320 ; http://n2t.net/addgene:73320 ; RRID:Addgene_73320)

  • For your References section:

    The CDK inhibitor CR8 acts as a molecular glue degrader that depletes cyclin K. Slabicki M, Kozicka Z, Petzold G, Li YD, Manojkumar M, Bunker RD, Donovan KA, Sievers QL, Koeppel J, Suchyta D, Sperling AS, Fink EC, Gasser JA, Wang LR, Corsello SM, Sellar RS, Jan M, Gillingham D, Scholl C, Frohling S, Golub TR, Fischer ES, Thoma NH, Ebert BL. Nature. 2020 Sep;585(7824):293-297. doi: 10.1038/s41586-020-2374-x. Epub 2020 Jun 3. 10.1038/s41586-020-2374-x PubMed 32494016
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