pJK142
(Plasmid #73317)

• Purpose: Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) as a fusion to Pas2p, with c-myc tag and His6-tag
• Depositing Lab: T. J. Kappock
• Publication: Additional fatty acid desaturase and acetylenase constructs (unpublished)

Backbone

• Vector backbone: pPICZA
• Backbone size w/o insert (bp): 3329
• Total vector size (bp): 5856
• Vector type: Yeast Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Bleocin (Zeocin), 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: delta-12 fatty acid acetylenase
• Alt name: vFAD2
• Species: Crepis alpina
• Mutation: synthetic gene. Encodes a glycine-204 to serine mutation in the Pas2 fusion domain
• GenBank ID: ABC00769.1
• Promoter: AOX1
• Tags / Fusion Proteins:
  • c-myc (C terminal on backbone)
  • His6 (C terminal on backbone)
  • Pas2p (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PmlI (not destroyed)
• 3′ cloning site: ApaI (not destroyed)
• 5′ sequencing primer: 5'-AOX1 primer
• 3′ sequencing primer: 3'-AOX primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry