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Addgene

pJK141
(Plasmid #73316)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73316 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPICZA
  • Backbone size w/o insert (bp) 3329
  • Total vector size (bp) 4617
  • Vector type
    Yeast Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Bleocin (Zeocin), 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    delta-12 fatty acid acetylenase
  • Alt name
    vFAD2
  • Species
    Crepis alpina
  • Mutation
    synthetic gene
  • GenBank ID
    ABC00769.1
  • Promoter AOX1
  • Tags / Fusion Proteins
    • c-myc (C terminal on backbone)
    • His6 (C terminal on backbone)
    • Pas2p signal sequence (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PmlI (not destroyed)
  • 3′ cloning site ApaI (not destroyed)
  • 5′ sequencing primer 5'-AOX1 primer
  • 3′ sequencing primer 3'-AOX primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK141 was a gift from T. J. Kappock (Addgene plasmid # 73316 ; http://n2t.net/addgene:73316 ; RRID:Addgene_73316)
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