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Addgene

pJK139
(Plasmid #73315)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73315 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPICZA
  • Backbone size w/o insert (bp) 3329
  • Total vector size (bp) 4743
  • Vector type
    Yeast Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Bleocin (Zeocin), 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Pas2p peroxisomal membrane targeting protein
  • Alt name
    peroxisomal biogenesis factor 3
  • Alt name
    PEX3_PICPA
  • Species
    Komagataella pastoris
  • Mutation
    Encodes a glycine-204 to serine mutation
  • GenBank ID
    CAA96530.1
  • Promoter AOX1
  • Tags / Fusion Proteins
    • c-myc (C terminal on backbone)
    • His6 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PmlI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer 5'-AOX1 primer
  • 3′ sequencing primer 3'-AOX primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK139 was a gift from T. J. Kappock (Addgene plasmid # 73315 ; http://n2t.net/addgene:73315 ; RRID:Addgene_73315)
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