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pTYB3-human PUM1-HD PUF531_Q867E-Q939E-C935S-Q1011E- C1007S-intein-CBD
(Plasmid #73306)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73306 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTYB3
  • Backbone manufacturer
    New England Biolabs
  • Backbone size w/o insert (bp) 7477
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    human PUM1 Pumilio homology domain
  • Alt name
    PUM1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1053
  • Mutation
    Q867E-Q939E-C935S-Q1011E-C1007S
  • Entrez Gene
    PUM1 (a.k.a. HSPUM, NEDMSF, PUMH, PUMH1, PUML1, SCA47)
  • Tag / Fusion Protein
    • intein (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site SapI (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer IntR
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTYB3-human PUM1-HD PUF531_Q867E-Q939E-C935S-Q1011E- C1007S-intein-CBD was a gift from Traci Hall (Addgene plasmid # 73306 ; http://n2t.net/addgene:73306 ; RRID:Addgene_73306)

  • For your References section:

    Engineering splicing factors with designed specificities. Wang Y, Cheong CG, Hall TM, Wang Z. Nat Methods. 2009 Nov;6(11):825-30. doi: 10.1038/nmeth.1379. Epub 2009 Oct 4. 10.1038/nmeth.1379 PubMed 19801992
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