pTYB3-human PUM1-HD MUT1-1 (Q867E)-intein-CBD
(Plasmid #73301)

• Purpose: Expresses mutant human PUM1-HD
• Depositing Lab: Traci Hall
• Publication: Cheong et al Proc Natl Acad Sci U S A. 2006 Sep 12;103(37):13635-9. Epub 2006 Sep 5.

Backbone

• Vector backbone: pTYB3
• Backbone manufacturer: New England Biolabs
• Backbone size w/o insert (bp): 7477
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: human PUM1 Pumilio homology domain
• Alt name: PUM1
• Species: H. sapiens (human)
• Insert Size (bp): 1053
• Mutation: Q867E
• Entrez Gene: PUM1 (a.k.a. HSPUM, NEDMSF, PUMH, PUMH1, PUML1, SCA47)
• Tag / Fusion Protein:
  • intein (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: SapI (destroyed during cloning)
• 5′ sequencing primer: T7
• 3′ sequencing primer: IntR

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry