pGolt-mCherry / pGolt3-mCherry
(Plasmid #73297)

• Purpose: Visualization of Dendritic Golgi Satellite
• Depositing Lab: Michael Kreutz
• Publication: Mikhaylova et al Cell Rep. 2015 Dec 30. pii: S2211-1247(15)01437-0. doi: 10.1016/j.celrep.2015.12.024.

Backbone

• Vector backbone: pmCherryN1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4773
• Total vector size (bp): 4896
• Modifications to backbone: This plasmid contains the multiple cloning site (MCS) of pmCherry-N1 vector.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: XL10-Gold Ultracompetent cells should be used for multiplication of plasmid.
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: ER export signal of Scap
• Alt name: SREBF chaperone (Scap)
• Species: Cricetulus griseus (Chinese hamster)
• Insert Size (bp): 36
• GenBank ID: NM_001244036 NP_001230965
• Promoter: CMV immediate early promoter
• Tag / Fusion Protein:
  • mCherry (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: BspEI (not destroyed)
• 3′ cloning site: BspEI (destroyed during cloning)
• 5′ sequencing primer: Forward primer for pEGFPN1 or pmCherryN1
• 3′ sequencing primer: Reverse primer for pmCherryN

Gene/Insert 2

• Gene/Insert name: ER export signal of Scap
• Alt name: SREBF chaperone (Scap)
• Species: Cricetulus griseus (Chinese hamster)
• Insert Size (bp): 36
• GenBank ID: NM_001244036 NP_001230965
• Promoter: CMV immediate early promoter
• Tag / Fusion Protein:
  • ER export (N terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: BspEI (destroyed during cloning)
• 3′ cloning site: BspEI (destroyed during cloning)
• 5′ sequencing primer: Forward primer for pEGFPN1 or pmCherryN1
• 3′ sequencing primer: Reverse primer for pmCherryN

Gene/Insert 3

• Gene/Insert name: TMD (trans-membrane domain) of Calneuron-2
• Alt name: calcium binding protein 7 (Cabp7)/C-terminal region of Calneuron-2
• Species: R. norvegicus (rat)
• Insert Size (bp): 87
• GenBank ID: NM_001007730 NP_001007731
• Entrez Gene: Cabp7 (a.k.a. rCaBP7)
• Promoter: CMV immediate early promoter
• Tag / Fusion Protein:
  • ER export (N terminal on insert)

Cloning Information for Gene/Insert 3

• Cloning method: Restriction Enzyme
• 5′ cloning site: BspEI (destroyed during cloning)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: Forward primer for pEGFPN1 or pmCherryN1
• 3′ sequencing primer: Reverse primer for pmCherryN

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry