pCfB2904(XI-3 MarkerFree)
(Plasmid #73276)

• Purpose: (Empty Backbone) EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-3 (Chr XI: 93378..94567)
• Depositing Lab: Irina Borodina
• Publication: Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.

Backbone

• Vector backbone: p0385 (pXI-3-URA3-DR)
• Backbone manufacturer: DTU
• Backbone size (bp): 4548
• Modifications to backbone: The pCfB2904 vector was created by amplifying the p0385 (pXI-3-URA3-DR) with primers that were designed to attach to either side of the selection markers, creating a fragment that no longer contained the marker. These fragments were then ligated to form the marker-less vectors. The PAM sites in the integration regions were removed by site-directed mutagenesis.
• Vector type: Yeast Expression, CRISPR, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme

Resource Information

• Supplemental Documents:
  • pCfB2904(XI-3 MarkerFree)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry