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Addgene

pJK103
(Plasmid #73265)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73265 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET23a
  • Backbone size w/o insert (bp) 3666
  • Total vector size (bp) 4052
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    BL21(DE3)pLysS
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    hypothetical acetic acid resistance protein B
  • Alt name
    aarB
  • Species
    Acetobacter aceti 1023
  • Promoter T7
  • Tag / Fusion Protein
    • His6 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Gene constructed from synthetic DNA.

Produces a form of a protein described as AarB in Fukaya M, Takemura H, Okumura H, Kawamura Y, Horinouchi S, Beppu T. Cloning of genes responsible for acetic acid resistance in Acetobacter aceti. J Bacteriol. 1990 Apr;172(4):2096-104. (PMID 2156811)

Does not appear to produce a soluble protein of the anticipated size.

Gene reannotated as SixA, on other strand, in Mullins EA, Francois JA, Kappock TJ. A specialized citric acid cycle requiring succinyl-coenzyme A (CoA):acetate CoA-transferase (AarC) confers acetic acid resistance on the acidophile Acetobacter aceti. J Bacteriol. 2008 Jul;190(14):4933-40. doi: 10.1128/JB.00405-08. (PMID 18502856)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK103 was a gift from T. J. Kappock (Addgene plasmid # 73265 ; http://n2t.net/addgene:73265 ; RRID:Addgene_73265)