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pcDNA3.1-KozATG-dH6.2-2XG4S-actin
(Plasmid #73263)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73263 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5382
  • Total vector size (bp) 7458
  • Modifications to backbone
    The NdeI site was removed from the CMV promoter
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    No special instructions.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KozATG-dH6.2-2XG4S-actin
  • Alt name
    dH6-actin
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    2070
  • Mutation
    The dH6.2 FAP has one cysteine in each domain changed to an alanine
  • Entrez Gene
    ACTB (a.k.a. BKRNS, BNS, BRWS1, CSMH, DDS1, PS1TP5BP1, THC8)
  • Promoter CMV
  • Tag / Fusion Protein
    • The FAP and actin are fused with 2 copies of a G4S linker

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMVF
  • 3′ sequencing primer BGHR
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit the Molecular Biosensor and Imaging Center website for current protocols and information about dyes to be used in combination with the proteins expressed from this plasmid.

http://pathways.mbic.cmu.edu/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-KozATG-dH6.2-2XG4S-actin was a gift from Marcel Bruchez (Addgene plasmid # 73263 ; http://n2t.net/addgene:73263 ; RRID:Addgene_73263)

  • For your References section:

    Localization microscopy using noncovalent fluorogen activation by genetically encoded fluorogen-activating proteins. Yan Q, Schwartz SL, Maji S, Huang F, Szent-Gyorgyi C, Lidke DS, Lidke KA, Bruchez MP. Chemphyschem. 2014 Mar 17;15(4):687-95. doi: 10.1002/cphc.201300757. Epub 2013 Nov 5. 10.1002/cphc.201300757 PubMed 24194371
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