BAZ2A
(Plasmid
#73261)
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PurposeBacterial expression for structure determination; may not be full ORF
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 73261 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET-SUMO
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameBAZ2A
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Alt nameBAZ2AA-c026
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Alt namegi|7304921
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SpeciesH. sapiens (human)
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Insert Size (bp)582
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Mutationpfam00628:PHD 1652-1696
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Entrez GeneBAZ2A (a.k.a. TIP5, WALp3)
- Promoter T7
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Tag
/ Fusion Protein
- His-6-SUMO (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7
- 3′ sequencing primer T7 terminal (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Use SF9 for protein expression. See also 4Q6F: http://www.thesgc.org/structures/4Q6F
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
BAZ2A was a gift from Nicola Burgess-Brown (Addgene plasmid # 73261 ; http://n2t.net/addgene:73261 ; RRID:Addgene_73261)