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Addgene

SPHK1
(Plasmid #73260)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73260 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFB-LIC-Bse
  • Backbone manufacturer
    SGC, Addgene Plasmid #26108
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SPHK1
  • Alt name
    SPHK1B-c012
  • Alt name
    gi|41282002
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1229
  • Entrez Gene
    SPHK1 (a.k.a. SPHK)
  • Promoter Polyhedrin
  • Tag / Fusion Protein
    • His-6-TEV (N terminal on backbone)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer fBac-1 (tattcataccgtcccacca)
  • 3′ sequencing primer fBac-2 (gggaggttttttaaagcaagtaaa)
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Use SF9 for protein expression. See also 4V24: http://www.thesgc.org/structures/4V24

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SPHK1 was a gift from Nicola Burgess-Brown (Addgene plasmid # 73260 ; http://n2t.net/addgene:73260 ; RRID:Addgene_73260)
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