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Addgene

CDK12
(Plasmid #73258)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73258 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFB-LIC-Bse
  • Backbone manufacturer
    SGC, Addgene Plasmid #26108
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CDK12
  • Alt name
    CRK7A-c020
  • Alt name
    gi|7706549
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1136
  • Mutation
    pfam00069: Pkinase 727-1020
  • Entrez Gene
    CDK12 (a.k.a. CRK7, CRKR, CRKRS)
  • Promoter Polyhedrin
  • Tag / Fusion Protein
    • His-6-TEV (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer fBac-1 (tattcataccgtcccacca)
  • 3′ sequencing primer fBac-2 (gggaggttttttaaagcaagtaaa)
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Use SF9 for protein expression. See also 4CXA: http://www.thesgc.org/structures/4CXA

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CDK12 was a gift from Nicola Burgess-Brown (Addgene plasmid # 73258 ; http://n2t.net/addgene:73258 ; RRID:Addgene_73258)
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