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Addgene

TP53BP1
(Plasmid #73252)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73252 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TP53BP1A
  • Alt name
    TP53BP1A-c002
  • Alt name
    gi|5032189
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    438
  • Entrez Gene
    TP53BP1 (a.k.a. 53BP1, TDRD30, p202, p53BP1)
  • Promoter T7
  • Tag / Fusion Protein
    • His6-Thrombin (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminal
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Use BL21(DE3) for protein expression. See also 4CRI: http://www.thesgc.org/structures/4CRI

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TP53BP1 was a gift from Nicola Burgess-Brown (Addgene plasmid # 73252 ; http://n2t.net/addgene:73252 ; RRID:Addgene_73252)
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