MAGEA3
(Plasmid #73246)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Nicola Burgess-Brown
• Publication: SGC Oxford (unpublished)

Backbone

• Vector backbone: pNH-TrxT
• Backbone manufacturer: SGC, Addgene Plasmid #26106
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: MAGEA3
• Alt name: MAGEA3A-c203
• Alt name: gi|4885467
• Species: H. sapiens (human)
• Insert Size (bp): 1070
• Mutation: pfam01454:MAGE 116-286
• Entrez Gene: MAGEA3 (a.k.a. CT1.3, HIP8, HYPD, MAGE3, MAGEA6)
• Promoter: T7
• Tag / Fusion Protein:
  • His6-Thioredoxin-TEV (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 terminal

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Use BL21(DE3)-R3-pRARE2 for protein expression. See also 4V0P: http://www.thesgc.org/structures/4V0P