AAK1
(Plasmid
#73244)
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PurposeBacterial expression for structure determination; may not be full ORF
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 73244 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepNIC-CTH0
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Backbone manufacturerSGC
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameAAK1
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Alt nameAAK1A-c046
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Alt namegi|29570780
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SpeciesH. sapiens (human)
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Insert Size (bp)1078
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Mutationpfam00069:Pkinase 47-307
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Entrez GeneAAK1
- Promoter T7
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Tag
/ Fusion Protein
- TEV-His6-Flag (C terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7 terminal (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Use BL21(DE3)-R3-lambda-PPase for protein expression. See also 4WSQ: http://www.thesgc.org/structures/4WSQ
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAK1 was a gift from Nicola Burgess-Brown (Addgene plasmid # 73244 ; http://n2t.net/addgene:73244 ; RRID:Addgene_73244)