RHOA
(Plasmid #73231)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Nicola Burgess-Brown
• Publication: SGC Oxford (unpublished)

Backbone

• Vector backbone: pNIC28-Bsa4
• Backbone manufacturer: SGC, Addgene Plasmid #26103
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: RHOA
• Alt name: RHOAA-c001
• Alt name: gi|10835049
• Species: H. sapiens (human)
• Insert Size (bp): 646
• Mutation: pfam00071:Ras 7-180
• Entrez Gene: RHOA (a.k.a. ARH12, ARHA, EDFAOB, RHO12, RHOH12)
• Promoter: T7
• Tag / Fusion Protein:
  • His-6-TEV (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 terminal

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Use BL21(DE3)-R3-pRARE2 for protein expression. See also 4D0N: http://www.thesgc.org/structures/4D0N