pEcCas
(Plasmid #73227)

• Purpose: Constitutive expression of cas9 and inducible expression of lambda RED and sgRNA
• Depositing Lab: Sheng Yang
• Publication: Li et al Acta Biochim Biophys Sin (Shanghai). 2021 Apr 15;53(5):620-627. doi: 10.1093/abbs/gmab036.

Backbone

• Vector backbone: pWM119
• Modifications to backbone: replaced Amp with Kan
• Vector type: Bacterial Expression, CRISPR ; Red recombinase

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: Cas9
• Alt name: pCASsac
• Species: S. pyogenes MGAS5005
• Promoter: native promoter

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: pENTR-R
• 3′ sequencing primer: AraC-F-New

Gene/Insert 2

• Gene/Insert name: sgRNA
• Species: Synthetic

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: unknown

Resource Information

• Supplemental Documents:
  • pCASsac
• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry