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pET21-10XHis-GST-HRV-dL5-Her1
(Plasmid #73215)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73215 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5387
  • Total vector size (bp) 7046
  • Modifications to backbone
    none
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    For expression use a strain that contains T7 RNA polymerase and trxB/gor double mutant such as Rosetta-gami(DE3)
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    EGFR
  • Alt name
    LH
  • Alt name
    FA
  • Species
    H. sapiens (human), Synthetic; Schistosoma japonicum
  • Insert Size (bp)
    1662
  • Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L91S and NP138
  • Entrez Gene
    EGFR (a.k.a. ERBB, ERBB1, ERRP, HER1, NISBD2, NNCIS, PIG61, mENA)
  • Promoter T7
  • Tags / Fusion Proteins
    • 10XHis-GST-HRV (N terminal on insert)
    • The dL5 FAP and Her1 affibody are fused with 8 amino acids between

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI and HindIII for FAP-Affibody (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 and GSTSeqF
  • 3′ sequencing primer T7term
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit the Molecular Biosensor and Imaging Center website for current protocols and information about dyes to be used in combination with the proteins expressed from this plasmid.

http://pathways.mbic.cmu.edu/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21-10XHis-GST-HRV-dL5-Her1 was a gift from Marcel Bruchez (Addgene plasmid # 73215 ; http://n2t.net/addgene:73215 ; RRID:Addgene_73215)

  • For your References section:

    Fluorogen activating protein-affibody probes: modular, no-wash measurement of epidermal growth factor receptors. Wang Y, Telmer CA, Schmidt BF, Franke JD, Ort S, Arndt-Jovin DJ, Bruchez MP. Bioconjug Chem. 2015 Jan 21;26(1):137-44. doi: 10.1021/bc500525b. Epub 2014 Dec 19. 10.1021/bc500525b PubMed 25490520
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