pET21-10XHis-GST-HRV-dL5
(Plasmid #73214)

• Purpose: Bacterial expression vector for dL5(E52D) protein with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP)
• Depositing Lab: Marcel Bruchez
• Publication: Wang et al Bioconjug Chem. 2015 Jan 21;26(1):137-44. doi: 10.1021/bc500525b. Epub 2014 Dec 19.

Backbone

• Vector backbone: pET21a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5384
• Total vector size (bp): 6500
• Modifications to backbone: none
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: For protein expression use a strain containing T7 RNA polymerase and trxB/gor double mutants such as Rosetta-gami(DE3)
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: EGFR
• Alt name: dL5** FAP
• Species: H. sapiens (human); Schistosoma japonicum
• Insert Size (bp): 1482
• Entrez Gene: EGFR (a.k.a. ERBB, ERBB1, ERRP, HER1, NISBD2, NNCIS, PIG61, mENA)
• Promoter: T7
• Tag / Fusion Protein:
  • 10XHis-GST-HRV (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7 and GSTSeqF
• 3′ sequencing primer: T7term

Resource Information

• Supplemental Documents:
  • pET21a-10XHis-GST-HRV-dL5(YW)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit the Molecular Biosensor and Imaging Center website for current protocols and information about dyes to be used in combination with the proteins expressed from this plasmid.

http://pathways.mbic.cmu.edu/