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Addgene

pET15b His6-ecDHFR (WT)
(Plasmid #73188)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73188 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET15b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5708
  • Total vector size (bp) 6183
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    folA dihydrofolate reductase
  • Alt name
    DHFR
  • Species
    E. coli
  • Insert Size (bp)
    486
  • Mutation
    none
  • GenBank ID
    AAC73159
  • Entrez Gene
    folA (a.k.a. b0048, ECK0049, tmr, tmrA)
  • Tag / Fusion Protein
    • His 6X (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15b His6-ecDHFR (WT) was a gift from Thomas Wandless (Addgene plasmid # 73188 ; http://n2t.net/addgene:73188 ; RRID:Addgene_73188)
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