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Addgene

pEF5B-FRT-control-APEX
(Plasmid #73187)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73187 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEF5B.FRT.DEST
  • Backbone size w/o insert (bp) 6833
  • Total vector size (bp) 7355
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    control-APEX
  • Alt name
    NPHP3[1-203.G2A]-GFP-APEX
  • Species
    M. musculus (mouse); Glycine max
  • Insert Size (bp)
    2253
  • Mutation
    changed glycine 2 to alanine
  • GenBank ID
    NP_082997 AAA61779.1
  • Entrez Gene
    Nphp3 (a.k.a. 3632410F03Rik, AI550417, C230078J01, D330020E01Rik, pcy)
  • Promoter EF1alpha
  • Tag / Fusion Protein
    • internal GFP tag

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    addgene

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

APEX sequence derived from glycine max according to: Rhee et al., Science, 2013

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF5B-FRT-control-APEX was a gift from Maxence Nachury (Addgene plasmid # 73187 ; http://n2t.net/addgene:73187 ; RRID:Addgene_73187)
  • For your References section:

    Proteomics of Primary Cilia by Proximity Labeling. Mick DU, Rodrigues RB, Leib RD, Adams CM, Chien AS, Gygi SP, Nachury MV. Dev Cell. 2015 Nov 23;35(4):497-512. doi: 10.1016/j.devcel.2015.10.015. Epub 2015 Nov 12. 10.1016/j.devcel.2015.10.015 PubMed 26585297