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PurposeExpresses "control"-APEX (cytosolic) in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 73187 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEF5B.FRT.DEST
- Backbone size w/o insert (bp) 6833
- Total vector size (bp) 7355
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Vector typeMammalian Expression
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecontrol-APEX
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Alt nameNPHP3[1-203.G2A]-GFP-APEX
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SpeciesM. musculus (mouse); Glycine max
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Insert Size (bp)2253
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Mutationchanged glycine 2 to alanine
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GenBank IDNP_082997 AAA61779.1
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Entrez GeneNphp3 (a.k.a. 3632410F03Rik, AI550417, C230078J01, D330020E01Rik, pcy)
- Promoter EF1alpha
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Tag
/ Fusion Protein
- internal GFP tag
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer T7 promotor
- 3′ sequencing primer BGH reverse (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byaddgene
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
APEX sequence derived from glycine max according to: Rhee et al., Science, 2013
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEF5B-FRT-control-APEX was a gift from Maxence Nachury (Addgene plasmid # 73187 ; http://n2t.net/addgene:73187 ; RRID:Addgene_73187) -
For your References section:
Proteomics of Primary Cilia by Proximity Labeling. Mick DU, Rodrigues RB, Leib RD, Adams CM, Chien AS, Gygi SP, Nachury MV. Dev Cell. 2015 Nov 23;35(4):497-512. doi: 10.1016/j.devcel.2015.10.015. Epub 2015 Nov 12. 10.1016/j.devcel.2015.10.015 PubMed 26585297