pEF5B-FRT-cilia-APEX
(Plasmid #73186)

• Purpose: Expresses cilia-APEX in mammalian cells
• Depositing Lab: Maxence Nachury
• Publication: Mick et al Dev Cell. 2015 Nov 23;35(4):497-512. doi: 10.1016/j.devcel.2015.10.015. Epub 2015 Nov 12.

Backbone

• Vector backbone: pEF5B.FRT.DEST
• Backbone size w/o insert (bp): 6833
• Total vector size (bp): 7355
• Vector type: Mammalian Expression
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cilia-APEX
• Alt name: NPHP3[1-203]-GFP-APEX
• Species: M. musculus (mouse); Glycine max
• Insert Size (bp): 2253
• GenBank ID: NP_082997 AAA61779.1
• Entrez Gene: Nphp3 (a.k.a. 3632410F03Rik, AI550417, C230078J01, D330020E01Rik, pcy)
• Promoter: EF1alpha
• Tag / Fusion Protein:
  • internal GFP tag

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: T7 promotor
• 3′ sequencing primer: BGH reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: addgene

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

APEX sequence derived from glycine max according to: Rhee et al., Science, 2013