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Addgene

pET15b His6-FKBPF36V
(Plasmid #73180)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73180 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET15b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5708
  • Total vector size (bp) 6063
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    FKBP(F36V)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    327
  • Mutation
    F36V
  • GenBank ID
    NM_000801
  • Entrez Gene
    FKBP1A (a.k.a. FKBP-12, FKBP-1A, FKBP1, FKBP12, PKC12, PKCI2, PPIASE)
  • Tag / Fusion Protein
    • His 6x (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15b His6-FKBPF36V was a gift from Thomas Wandless (Addgene plasmid # 73180 ; http://n2t.net/addgene:73180 ; RRID:Addgene_73180)

  • For your References section:

    Ligand-switchable substrates for a ubiquitin-proteasome system. Egeler EL, Urner LM, Rakhit R, Liu CW, Wandless TJ. J Biol Chem. 2011 Sep 9;286(36):31328-36. doi: 10.1074/jbc.M111.264101. Epub 2011 Jul 15. 10.1074/jbc.M111.264101 PubMed 21768107
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