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PurposeExpressing iBlueberry and mCherry in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 73176 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepN1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameLifeAct-iBlueberry
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Mutationmutation E177Q in iBlueberry
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1_LifeAct-iBlueberry was a gift from Xiaokun Shu (Addgene plasmid # 73176 ; http://n2t.net/addgene:73176 ; RRID:Addgene_73176) -
For your References section:
Rational design of a monomeric and photostable far-red fluorescent protein for fluorescence imaging in vivo. Yu D, Dong Z, Gustafson WC, Ruiz-Gonzalez R, Signor L, Marzocca F, Borel F, Klassen MP, Makhijani K, Royant A, Jan YN, Weiss WA, Guo S, Shu X. Protein Sci. 2016 Feb;25(2):308-15. doi: 10.1002/pro.2843. Epub 2015 Dec 31. 10.1002/pro.2843 PubMed 26549191