pDZ645 pKAN 1x-mCherry-12xPP7 V4
(Plasmid #73173)

• Purpose: Floxed Kanamycin (G418) selection maker; 3' tagging vector used to add 1x-mCherry followed by 12 repetitive PP7 version 4 RNA stem-loops to gene of interest
• Depositing Lab: Daniel Zenklusen
• Publication: Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40. doi: 10.1083/jcb.201503070.

Backbone

• Vector backbone: pKAN
• Backbone size w/o insert (bp): 4727
• Total vector size (bp): 5951
• Vector type: Mammalian Expression, Yeast Expression
• Selectable markers: Kanamycin (yeast selection with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: 1x-mCherry
• Species: Synthetic
• Insert Size (bp): 1224

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BglII (not destroyed)
• 5′ sequencing primer: TGT AAA ACG ACG GCC AGT

Resource Information

• Supplemental Documents:
  • pDZ645 GenBank
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry