pET11_DHNTPase
(Plasmid #73154)

• Purpose: Expresses in E. coli, E. coli Dihydroneopterin triphosphate pyrophosphatase
• Depositing Labs: Maurice Bessman, Sandra Gabelli
• Publication: Gabelli et al Structure. 2007 Aug;15(8):1014-22.

Backbone

• Vector backbone: pET11b
• Backbone manufacturer: Novagen
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Four liters of LB medium containing 100 mg/ml ampicillin were inoculated with HMS174(DE3):pETorf17, grown at 37 °C to an A600 of 1.0, induced with 1 mM IPTG, and grown for an additional 2.5
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: ORF17_DHNTPase_NUD
• Alt name: DHNTPase
• Alt name: nudB
• Alt name: NC_000913.3
• Species: Escherichia coli
• GenBank ID: NC_000913.3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: I received this clone from Dr. Maurice J. Bessman

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry