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Addgene

pET11_DHNTPase
(Plasmid #73154)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73154 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET11b
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Four liters of LB medium containing 100 mg/ml ampicillin were inoculated with HMS174(DE3):pETorf17, grown at 37 °C to an A600 of 1.0, induced with 1 mM IPTG, and grown for an additional 2.5
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ORF17_DHNTPase_NUD
  • Alt name
    DHNTPase
  • Alt name
    nudB
  • Alt name
    NC_000913.3
  • Species
    Escherichia coli
  • GenBank ID
    NC_000913.3

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    I received this clone from Dr. Maurice J. Bessman

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET11_DHNTPase was a gift from Maurice Bessman & Sandra Gabelli (Addgene plasmid # 73154 ; http://n2t.net/addgene:73154 ; RRID:Addgene_73154)

  • For your References section:

    Structure and function of the E. coli dihydroneopterin triphosphate pyrophosphatase: a Nudix enzyme involved in folate biosynthesis. Gabelli SB, Bianchet MA, Xu W, Dunn CA, Niu ZD, Amzel LM, Bessman MJ. Structure. 2007 Aug;15(8):1014-22. 10.1016/j.str.2007.06.018 PubMed 17698004
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