pCTCON2 Aga2P-HRP
(Plasmid #73152)

• Purpose: Full-length horseradish peroxidase (HRP) fused to the C-terminus of the yeast mating protein Aga2P. Galactose-inducible expression.
• Depositing Lab: Alice Ting
• Publication: Martell et al Nat Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563.

Backbone

• Vector backbone: pCTCON2
• Backbone size w/o insert (bp): 55791
• Total vector size (bp): 7171
• Vector type: Yeast Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Aga2P-HRP
• Species: S. cerevisiae (budding yeast), Synthetic
• Insert Size (bp): 1380
• Promoter: GAL1
• Tags / Fusion Proteins:
  • HA tag
  • myc tag

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: gactacgctctgcaggagaacttg

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

HRP = full-length horseradish peroxidase.

This vector contains the following features:
EcoRI-Aga2P-HA-TEVs-15 aa linker-NheI-HRP-3 aa linker-BamHI-myc-stop-XhoI

GAL1 promoter (galactose inducible)
15 aa linker: GGGGSGGGGSGGGGS
3 aa linker: GSG
HA: YPYDVPDYA
myc: EQKLISEEDL
TEVs: ENLYFQG, cleavage recognition site for TEV protease

This construct is derived from the previously reported Aga2P-HRP construct (Lam et. al. Nature Methods, 12, 51-54, 2015), except the codon optimization of the synthetic HRP gene is different in this construct.