pDisplay sHRPb-KDEL
(Plasmid #73150)

• Purpose: Small fragment of split HRP targeted for expression in the lumen of the endoplasmic reticulum (ER). CMV promoter.
• Depositing Lab: Alice Ting
• Publication: Martell et al Nat Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563.

Backbone

• Vector backbone: pDisplay
• Backbone size w/o insert (bp): 5208
• Total vector size (bp): 5643
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: sHRPb-KDEL
• Species: Synthetic
• Insert Size (bp): 435
• Promoter: CMV
• Tag / Fusion Protein:
  • V5 tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: AscI (not destroyed)
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

sHRPb is the small split HRP fragment. It consists of amino acids 214-308 of horseradish peroxidase (HRP) with the following 2 mutations: N255D, L299R

The insert contains the following features:
EcoRI-Ig k-chain ss-BglII-V5 epitope tag-sHRPb-3 aa linker-KDEL-Stop-AscI

V5 epitope tag: GKPIPNPLLGLDST
3 aa linker: GSG