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Addgene

pLPC myc-mRAP1 I312R
(Plasmid #73137)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73137 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLPC
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 7800
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rap1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1300
  • Mutation
    isoleucine changed to an arginine at amino acid 312
  • Entrez Gene
    Terf2ip (a.k.a. Rap1)
  • Tag / Fusion Protein
    • myc (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLPC myc-mRAP1 I312R was a gift from Agnel Sfeir (Addgene plasmid # 73137 ; http://n2t.net/addgene:73137 ; RRID:Addgene_73137)

  • For your References section:

    Nontelomeric role for Rap1 in regulating metabolism and protecting against obesity. Yeung F, Ramirez CM, Mateos-Gomez PA, Pinzaru A, Ceccarini G, Kabir S, Fernandez-Hernando C, Sfeir A. Cell Rep. 2013 Jun 27;3(6):1847-56. doi: 10.1016/j.celrep.2013.05.032. Epub 2013 Jun 20. 10.1016/j.celrep.2013.05.032 PubMed 23791522
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