pLPC Flag-mRAP1
(Plasmid
#73135)
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PurposeExpression of Flag-tagged mRAP1
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 73135 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLPC
- Backbone size w/o insert (bp) 5500
- Total vector size (bp) 7800
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRap1
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SpeciesM. musculus (mouse)
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Insert Size (bp)1300
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Entrez GeneTerf2ip (a.k.a. Rap1)
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Tag
/ Fusion Protein
- Flag (N terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Additional reference: Cell Rep. 2013 Jun 27;3(6):1847-56.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLPC Flag-mRAP1 was a gift from Titia de Lange & Agnel Sfeir (Addgene plasmid # 73135 ; http://n2t.net/addgene:73135 ; RRID:Addgene_73135) -
For your References section:
Loss of Rap1 induces telomere recombination in the absence of NHEJ or a DNA damage signal. Sfeir A, Kabir S, van Overbeek M, Celli GB, de Lange T. Science. 2010 Mar 26;327(5973):1657-61. doi: 10.1126/science.1185100. 10.1126/science.1185100 PubMed 20339076