pcDNA 3.1(Hygro) myc-hPolQ-Flag
(Plasmid #73132)

• Purpose: To express in mammalian cells human PolQ
• Depositing Lab: Agnel Sfeir
• Publication: Mateos-Gomez et al Nature. 2015 Feb 12;518(7538):254-7. doi: 10.1038/nature14157. Epub 2015 Feb 2.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5607
• Total vector size (bp): 13416
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Polymerase theta
• Species: H. sapiens (human)
• Insert Size (bp): 7797
• Entrez Gene: POLQ (a.k.a. PRO0327)
• Promoter: cmv promoter
• Tags / Fusion Proteins:
  • Flag (C terminal on insert)
  • myc (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: TAATACGACTCACTATAGG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Proc Natl Acad Sci U S A. 2010 Jul 27;107(30):13390-5. doi: 10.1073/pnas.0910759107. Epub 2010 Jul 12. DNA polymerase theta up-regulation is associated with poor survival in breast cancer, perturbs DNA replication, and promotes genetic instability. Lemée F1, Bergoglio V, Fernandez-Vidal A, Machado-Silva A, Pillaire MJ, Bieth A, Gentil C, Baker L, Martin AL, Leduc C, Lam E, Magdeleine E, Filleron T, Oumouhou N, Kaina B, Seki M, Grimal F, Lacroix-Triki M, Thompson A, Roché H, Bourdon JC, Wood RD, Hoffmann JS, Cazaux C.
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

We added a myc tag to the N-terminus of the gene