CD47-CD4d3+4-bio
(Plasmid #73121)

• Purpose: EXPRESs plasmid for human erythrocyte surface proteins encoding CD47 with rat CD4d3+4-bio
• Depositing Lab: Gavin Wright
• Publication: Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi: 10.1038/nature10606.

Backbone

• Vector backbone: pTT3
• Backbone manufacturer: Yves Durocher, NRC
• Backbone size w/o insert (bp): 6000
• Total vector size (bp): 7001
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CD47 antigen
• Species: H. sapiens (human)
• GenBank ID:
• Entrez Gene: CD47 (a.k.a. IAP, MER6, OA3)
• Promoter: CMV
• Tags / Fusion Proteins:
  • ratCD4d3+4 (C terminal on insert)
  • biotinylation peptide (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: AscI (not destroyed)
• 5′ sequencing primer: CMW fwd

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pTT3 was obtained from Yves Durocher, National Research Council of Canada-Biotechnology Research Institute

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry