pcDNA3.1_hE2F7mg_2ntmut
(Plasmid
#73074)
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Purposehuman E2F7 minigene (exons 11,12,13/introns cassette) with mutated snord27 binding site
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 73074 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1V5hisTOPO
- Total vector size (bp) 9701
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameE2F7
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SpeciesH. sapiens (human)
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Insert Size (bp)4178
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Mutationpartial gene, exons 11,12,13 spaced by introns, 2nt mutation in SNORD27 binding site
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Entrez GeneE2F7
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer AAACCCTCAGATTCCACAG
- 3′ sequencing primer ACATCCGGGACTCTCAG (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1_hE2F7mg_2ntmut was a gift from Stefan Stamm (Addgene plasmid # 73074 ; http://n2t.net/addgene:73074 ; RRID:Addgene_73074) -
For your References section:
Dual function of C/D box small nucleolar RNAs in rRNA modification and alternative pre-mRNA splicing. Falaleeva M, Pages A, Matuszek Z, Hidmi S, Agranat-Tamir L, Korotkov K, Nevo Y, Eyras E, Sperling R, Stamm S. Proc Natl Acad Sci U S A. 2016 Mar 22;113(12):E1625-34. doi: 10.1073/pnas.1519292113. Epub 2016 Mar 8. 10.1073/pnas.1519292113 PubMed 26957605