pcDNA3.1_SNORD27h_2ntmut
(Plasmid
#73067)
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Purposeexpression clone for human mutated SNORD27 (C/D box snoRNA U27)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 73067 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1(+)V5 TOPO
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSNORD27
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SpeciesH. sapiens (human)
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Mutation2 nt mutation in AS box that binds E2F7 pre-mRNA
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Entrez GeneE2F7
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Tag
/ Fusion Protein
- no tag
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer ATGTTCAGCCCACAAGAGC
- 3′ sequencing primer CTGTATTCACCCTGGGAGG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1_SNORD27h_2ntmut was a gift from Stefan Stamm (Addgene plasmid # 73067 ; http://n2t.net/addgene:73067 ; RRID:Addgene_73067) -
For your References section:
Dual function of C/D box small nucleolar RNAs in rRNA modification and alternative pre-mRNA splicing. Falaleeva M, Pages A, Matuszek Z, Hidmi S, Agranat-Tamir L, Korotkov K, Nevo Y, Eyras E, Sperling R, Stamm S. Proc Natl Acad Sci U S A. 2016 Mar 22;113(12):E1625-34. doi: 10.1073/pnas.1519292113. Epub 2016 Mar 8. 10.1073/pnas.1519292113 PubMed 26957605