pCMV14_NHP2L1(15.5kD)
(Plasmid #73065)

• Purpose: expression clone for human NHP2L1 (15.5kD) with C-terminal 3xFLAG-tag
• Depositing Lab: Stefan Stamm
• Publication: Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;113(12):E1625-34. doi: 10.1073/pnas.1519292113. Epub 2016 Mar 8.

Backbone

• Vector backbone: pCMV14
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: NHP2L1 (15.5kD)
• Alt name: SNU13 homolog, small nuclear ribonucleoprotein (U4/U6.U5)
• Species: H. sapiens (human)
• Insert Size (bp): 1587
• Entrez Gene: SNU13 (a.k.a. 15.5K, FA-1, FA1, NHP2L1, NHPX, OTK27, SNRNP15-5, SPAG12, SSFA1)
• Tag / Fusion Protein:
  • 3xFLAG (C terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: tcggtaccagtcgacATGACTGAGGCTGATGTG
• 3′ sequencing primer: ccgggatcctctagaGACTAAGAGCCTTTCAATG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry