pCMV14_NOP58
(Plasmid #73063)

• Purpose: expression clone for human NOP58 with C-terminal 3xFLAG-tag
• Depositing Lab: Stefan Stamm
• Publication: Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;113(12):E1625-34. doi: 10.1073/pnas.1519292113. Epub 2016 Mar 8.

Backbone

• Vector backbone: pCMV14
• Total vector size (bp): 7897
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: NOP58 ribonucleoprotein
• Species: H. sapiens (human)
• Insert Size (bp): 1587
• Entrez Gene: NOP58 (a.k.a. HSPC120, NOP5, NOP5/NOP58)
• Promoter: CMV
• Tag / Fusion Protein:
  • 3xFLAG (C terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: tcggtaccagtcgacATGTTGGTGCTGTTTGAAAC
• 3′ sequencing primer: ccgggatcctctagaATCCTCGTTCTCTCTCTTTTTTTTC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry